UDP-galactopyranose Mutase from Aspergillus fumigatus

Description : This is the first reported structures of UGM from a eukaryotic pathogen. We have determined the crystal structures of UGM with and without substrates. Additionally, our crystal structures show rearrangements of the active site due to the oxidation state of the flavin cofactor. We have also determined the crystals structures of mutant UGMs with substrate bound.

Reference : J. Biol. Chem. (2012) 298: 10780-10790. van Straaten, K.E., Routier, F.H., and Sanders, D.A.R.

Inositol dehydrogenase from Bacillus subtilis

Description : Inositol dehydrogenase (IDH) catalyzes the conversion of 2-myo-Inositol to inosose, using NADH. We have determined the crystal structures of IDH with and without cofactor and substrate. We have also determined the crystals structures of an inactive mutant (K97V) with and without substrates/cofactors

Reference : Biochemical J. (2010) 432: 237-247. van Straaten, K.E., Zheng, H., Palmer, D.R.J., and Sanders, D.A.R.

UDP-galactopyranose mutase from Deinococcus radiodurans complexed with the Phosphonate analogue of UDP-Galp

Description : This structure represents the first structure of UDP-galactopyranose mutase complexed with an inhibitor. The phosphonate analogue (anomeric oxygen replace with a carbon) is a poor inhibitor and the structure offers an explanation for this poor inhibition.

Reference : J. Mol. Biol. (2010) 403: 578-590. Karunan Partha, S., Sadeghi-Khomami, A., Slowski, K., Kotake, T. , Thomas, N.R. Jakeman, D.L. and Sanders, D.A.R.

Thioredoxin reductase from Deinococcus radiodurans

Description : Thioredoxin reductase is one of the proteins responsible for maintaining the proper redox state of the cytoplasm of cells. We have determiend the structure of TrxR and characterized its kinetic parameters. We have also examined the ability of the Trx system from D. radiodurans to interact with the E.coli Trx system.

Reference : J. Bacteriology (2010) 192: 494-501. Obiero, J., Pittet, V., Bonderoff, S.A. and Sanders, D.A.R.

UDP-galactopyranose mutase from Deinococcus radiodurans

Description : UDP-galactopyranose mutase catalyzes an unprecedented interconversion of a 6 membered ring with a 5 membered ring. The product is an essential component of the cell wall of pathogenic micro-organisms, making this an ideal target for novel drug design. We have solved the structure of UGM complexed with substrates and with the flavin (FAD) oxidized and reduced.

Reference : J. Mol. Biol. (2009) 394: 864-877. Karunan Partha, S., van Straaten, K., Sanders, D.A.R.

Thioredoxin reductase from Helicobacter pylori

Description : Thioredoxin reductase is one of the proteins responsible for maintaining the proper redox state of the cytoplasm of cells.

Reference : Structure deposited October 2009. Obiero, J., van Straaten, K., Sanders, D.A.R.

S-Formylglutathione Hydrolase from Agrobacterium tumefaciens

Description : S-Formylgutathione hydrolase catalyzes the cleavage of S-formylglutathione into formate and glutathione. This family of proteins is proposed to be part of the glutatione-dependent formadlehyde oxidation pathway. Unlike other members of this family, SFGH from A.tumifaciens shows a preference towards medium chain esters.

Reference : Protein Science (2009) 18: 2196-2202. van Straaten, K.E., Gonzalez, C.F., Valladares, R.B., Xu, X., Savchenko, A.V. and Sanders, D.A.R.

PA1607 from Pseudomonas aureginosa

Description : This small protein has an unknown function, but is similar to the MarR family of transcription regulators.

Reference : Protein Science (2007) 16: 543-549. Sieminska, E.A.L., Xu, X., Savchenko, A., and Sanders, D.A.R.

PA3566 from Pseudomonas aureginosa

Description : This small protein has an unknown function, but is similar to small monooxygenases.

Reference : Proteins : Struct., Funct. & Bioinfo. (2005) 61: 209-212. Sanders, D.A.R., Walker, J.R., Skarina, T. and Savchenko, A.